What is the difference between the cells that resulting from mitosis and meiosis?

Your body is made up of trillions of cells that all originate from just one—a fertilized egg. The massive multiplication of cells after conception is possible thanks to cell division, which occurs when one cell splits into two. Cell division not only enables growth but also replaces damaged or dead cells and makes reproduction possible. There are two kinds of cell division: mitosis and meiosis.

Mitosis is shown on the left, and meiosis is shown on the right. Credit: Judith Stoffer. Click to enlarge

What’s the Difference?

Mitosis produces two genetically identical “daughter” cells from a single “parent” cell, whereas meiosis produces cells that are genetically unique from the parent and contain only half as much DNA. Most cells in the body regularly go through mitosis, but some do so more often than others. For instance, those that line the stomach replace themselves after just a few days because they’re subjected to strong digestive acids. In contrast, liver cells may wait up to a year to replace themselves. There are also a few types that last a lifetime without dividing, such as some nerve cells and cells that make up the lens of the eye. Meiosis occurs only in the production of sperm and egg cells for sexual reproduction.

The Phases of Cell Division

Before either mitosis or meiosis occurs, cells go through a preparatory process called interphase, where they grow and make a copy of their genetic information.

Mitosis has six phases apart from interphase. The first five phases divide the nucleus and its genetic information in half, while the final step splits the entire parent cell into two identical daughter cells. The phases of mitosis are:

1. Prophase: Chromosomes, which contain genetic information, condense and prepare to attach to the spindle—a cellular machine that moves chromosomes during cell division. 
2. Prometaphase: The nuclear membrane—a structure that typically contains the chromosomes—breaks apart, the spindle forms, and chromosomes attach to its strong, hollow fibers.
3. Metaphase: Chromosomes align along the spindle’s center. 
4. Anaphase: Chromosomes pull apart and move toward the spindle’s poles, which also move apart.
5. Telophase: New nuclear envelopes form around the two separated sets of chromosomes. 
6. Cytokinesis: Cells divide. 

Meiosis has similar steps to mitosis but with two sets of divisions. The first division results in two cells that each have two sets of chromosomes, like in mitosis. The second division creates four cells that each contain one set of chromosomes, because the genetic information isn’t copied a second time. One unique feature of meiosis, which takes place during the first round of prophase (prophase I), is a process called crossing over. DNA is mixed between matching chromosomes from the different parents, increasing the genetic diversity.

NIGMS-Funded Cell Division Research

Many scientists who are supported by NIGMS study cell division. Some of these researchers are investigating how cells:

• Maintain normal cell division through aligning and separating chromosomes via the spindle during mitosis and meiosis
• Prevent genetic errors from being introduced during crossing over in meiosis
• Decide to either grow and divide or enter a resting phase, which is important in understanding diseases caused by excessive or insufficient cell division

This post is a great supplement to Pathways: The Regeneration Issue.

Our bodies may not regenerate like some of the research organisms described in Pathways, but they can heal and replace damaged cells through mitosis—the subject of this post.

Although mitosis and meiosis both involve cell division, they transmit genetic material in very different ways. What happens when either of these processes goes awry?

Genetics owes a great debt to good drawing. Handmade graphic depictions of mitotic chromosomes by Walther Flemming (Figure 1) and meiotic chromosomes by Walter Sutton provided an early record of the physical path of chromosomes during cell division. The physical movement of chromosomes could then be correlated with cells' patterns of genetic inheritance. (The idea that genes were carried on cytological structures is now known as the chromosome theory.) Using such methods, researchers determined that although mitosis and meiosis are both forms of cell division, the results of these processes are actually quite different.

Gene Transmission in Mitosis

Mitosis occurs in somatic cells; this means that it takes place in all types of cells that are not involved in the production of gametes. Prior to each mitotic division, a copy of every chromosome is created; thus, following division, a complete set of chromosomes is found in the nucleus of each new cell. Indeed, apart from random mutations, each successive duplicate cell will have the same genetic composition as its parent, due to the inheritance of the same chromosome set and similar biological environment. This works well for replacing damaged tissue or for growth and expansion from an embryonic state.

Because the genes contained in the duplicate chromosomes are transferred to each successive cellular generation, all mitotic progeny are genetically similar. However, there are exceptions. For example, there are genetic variations that arise in clonal species, such as bacteria, due to spontaneous mutations during mitotic division. Furthermore, chromosomes are sometimes replicated multiple times without any accompanying cell division. This occurs in the cells of Drosophila larvae salivary glands, for example, where there is a high metabolic demand. The chromosomes there are called polytene chromosomes, and they are extremely large compared to chromosomes in other Drosophila cells. These chromosomes replicate by undergoing the initial phases of mitosis without any cytokinesis (Figure 2). Therefore, the same cell contains thick arrangements of duplicate chromosomes side by side, which look like strands of very thick rope. Scientists believe that these chromosomes are hyper-replicated to allow for the rapid and copious production of certain proteins that help larval growth and metamorphosis (Gilbert, 2008).

Gene Transmission in Meiosis

Figure 2: Examples of polytene chromosomes

Pairing of homologous chromatids results in hundreds to thousands of individual chromatid copies aligned tightly in parallel to produce giant, "polytene" chromosomes.

© 2007 Novikov, D. et al. High-pressure treatment of polytene chromosomes improves structural resolution. Nature Methods 4, 483 (2007). All rights reserved.

Although he did not know it, Walther Flemming actually observed spermatozoa undergoing meiosis in 1882, but he mistook this process for mitosis. Nonetheless, Flemming did notice that, unlike during regular cell division, chromosomes occurred in pairs during spermatozoan development. This observation, followed in 1902 by Sutton's meticulous measurement of chromosomes in grasshopper sperm cell development, provided definitive clues that cell division in gametes was not just regular mitosis. Sutton demonstrated that the number of chromosomes was reduced in spermatozoan cell division, a process referred to as reductive division. As a result of this process, each gamete that Sutton observed had one-half the genetic information of the original cell. A few years later, researchers J. B. Farmer and J. E. S. Moore reported that this process—otherwise known as meiosis—is the fundamental means by which animals and plants produce gametes (Farmer & Moore, 1905).

The greatest impact of Sutton's work has far more to do with providing evidence for Mendel's principle of independent assortment than anything else. Specifically, Sutton saw that the position of each chromosome at the midline during metaphase was random, and that there was never a consistent maternal or paternal side of the cell division. Therefore, each chromosome was independent of the other. Thus, when the parent cell separated into gametes, the set of chromosomes in each daughter cell could contain a mixture of the parental traits, but not necessarily the same mixture as in other daughter cells.

To illustrate this concept, consider the variety derived from just three hypothetical chromosome pairs, as shown in the following example (Hirsch, 1963). Each pair consists of two homologues: one maternal and one paternal. Here, capital letters represent the maternal chromosome, and lowercase letters represent the paternal chromosome:

• Pair 1: A and a
• Pair 2: B and b
• Pair 3: C and c

When these chromosome pairs are reshuffled through independent assortment, they can produce eight possible combinations in the resulting gametes:

• A B C
• A B c
• A b c
• A b C
• a B C
• a B c
• a b C
• a b c

A mathematical calculation based on the number of chromosomes in an organism will also provide the number of possible combinations of chromosomes for each gamete. In particular, Sutton pointed out that the independence of each chromosome during meiosis means that there are 2n possible combinations of chromosomes in gametes, with "n" being the number of chromosomes per gamete. Thus, in the previous example of three chromosome pairs, the calculation is 23, which equals 8. Furthermore, when you consider all the possible pairings of male and female gametes, the variation in zygotes is (2n)2, which results in some fairly large numbers.

But what about chromosome reassortment in humans? Humans have 23 pairs of chromosomes. That means that one person could produce 223 different gametes. In addition, when you calculate the possible combinations that emerge from the pairing of an egg and a sperm, the result is (223)2 possible combinations. However, some of these combinations produce the same genotype (for example, several gametes can produce a heterozygous individual). As a result, the chances that two siblings will have the same combination of chromosomes (assuming no recombination) is about (3/8)23, or one in 6.27 billion. Of course, there are more than 23 segregating units (Hirsch, 2004).

While calculations of the random assortment of chromosomes and the mixture of different gametes are impressive, random assortment is not the only source of variation that comes from meiosis. In fact, these calculations are ideal numbers based on chromosomes that actually stay intact throughout the meiotic process. In reality, crossing-over between chromatids during prophase I of meiosis mixes up pieces of chromosomes between homologue pairs, a phenomenon called recombination. Because recombination occurs every time gametes are formed, we can expect that it will always add to the possible genotypes predicted from the 2n calculation. In addition, the variety of gametes becomes even more unpredictable and complex when we consider the contribution of gene linkage. Some genes will always cosegregate into gametes if they are tightly linked, and they will therefore show a very low recombination rate. While linkage is a force that tends to reduce independent assortment of certain traits, recombination increases this assortment. In fact, recombination leads to an overall increase in the number of units that assort independently, and this increases variation.

While in mitosis, genes are generally transferred faithfully from one cellular generation to the next; in meiosis and subsequent sexual reproduction, genes get mixed up. Sexual reproduction actually expands the variety created by meiosis, because it combines the different varieties of parental genotypes. Thus, because of independent assortment, recombination, and sexual reproduction, there are trillions of possible genotypes in the human species.

Aberrations That Alter Chromosome Number

During cell division, chromosomes sometimes disappear. This occurs when there is some aberration in the centromere, and spindle fibers cannot attach to the chromosome to segregate it to distal poles of the cell. Consequently, the lost chromosome never properly groups with others into a new nuclear envelope, and it is left in the cytoplasm, where it will not be transcribed. Also, chromosomes don't always separate equally into daughter cells. This sometimes happens in mitosis, when sister chromatids fail to separate during anaphase. One daughter cell thus ends up with more chromosomes in its nucleus than the other. Likewise, abnormal separation can occur in meiosis when homologous pairs fail to separate during anaphase I. This also results in daughter cells with different numbers of chromosomes. The phenomenon of unequal separation in meiosis is called nondisjunction. If nondisjunction causes a missing chromosome in a haploid gamete, the diploid zygote it forms with another gamete will contain only one copy of that chromosome from the other parent, a condition known as monosomy. Conversely, if nondisjunction causes a homologous pair to travel together into the same gamete, the resulting zygote will have three copies, a condition known as trisomy (Figure 3). The term "aneuploidy" applies to any of these conditions that cause an unexpected chromosome number in a daughter cell.

Figure 3: Nondisjunction results in daughter cells with unusual chromosome numbers.

Nondisjunction, in which chromosomes fail to separate equally, can occur in meiosis I (first row), meiosis II (second row), and mitosis (third row). These unequal separations can produce daughter cells with unexpected chromosome numbers, called aneuploids. When a haploid gamete does not receive a chromosome during meiosis as a result of nondisjunction, it combines with another gamete to form a monosomic zygote. When a gamete receives a complete homologous chromosome pair as a result of nondisjunction, it combines with another gamete to form a trisomic zygote.

© 2014 Adapted from Pierce, Benjamin. Genetics: A Conceptual Approach, 2nd ed. All rights reserved.

© 2014 Adapted from Pierce, Benjamin. Genetics: A Conceptual Approach, 2nd ed. All rights reserved.

Some of the earliest evidence of aneuploidy came from observations of jimsonweed. In 1913, Albert Francis Blakeslee noticed that some jimsonweed seed pods showed strange deviations from the expected spherical shape. In fact, Blakeslee observed 12 different types of deviations from the expected pod shape (Figure 4), and they occurred in the pattern of a dominant trait. Interestingly, when an odd-shaped pod plant was backcrossed with itself, only 25% of the offspring retained the form, which is a much smaller outcome than what is expected from a dominant trait self-cross. This inheritance pattern was puzzling to Blakeslee, and it wasn't until 13 years later that his colleague John Belling finally explained its chromosomal basis (Belling & Blakeslee, 1926). Belling showed that the jimsonweed has 12 chromosome pairs, each of which could show trisomy. Each case of trisomy produces a unique phenotype. The plants with oddly shaped pods had an unusually low dominant trait frequency when self-crossed because the effect of nondisjunction was sometimes eliminated in the gametes. In the odd-shaped forms with trisomy, the extra chromosome was shed during meiosis about 50% of the time, so not all gametes carried the aberrant number of chromosomes. Also, those pollen grains carrying the trisomy were not as successful at fertilization. The jimsonweed trisomy is an interesting example of how aneuploidy does not transfer easily to successive generations and can be screened out by the meiotic process that it emerged from.

Aneuploidy can also occur in humans. For instance, the underlying causes of Klinefelter's syndrome and Turner's syndrome are errors in sex chromosome number, and Down syndrome is caused by trisomy of chromosome 21. However, the severity of phenotypic abnormalities can vary among different types of aneuploidy. In addition, aneuploidy is rarely transferred to subsequent generations, because this condition impairs the production of gametes.

Overall, the inheritance of odd chromosome number arises from errors in segregation during chromosome replication. Often, it is these very exceptions or modifications of expected patterns in mitosis and meiosis that enrich our understanding of how the transfer of chromosomes is regulated from one generation to the next.

References and Recommended Reading

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Belling, J., & Blakeslee, A. F. On the attachment of non-homologous chromosomes at the reduction division in certain 25-chromosome daturas. Proceedings of the National Academy of Sciences 12, 7–11 (1926)

Farmer, J. B., & Moore, J. E. S. On the maiotic phase (reduction divisions) in animals and plants. Quarterly Journal of Microscopical Science 48, 489–557 (1905)

Gilbert, S. F. Developmental Biology Companion Website (Sunderland, MA, Sinauer, 2010)

Hirsch, J. Behavior genetics and individuality understood. Science 42, 1436–1442 (1963) doi:10.1126/science.142.3598.1436

---. Uniqueness, diversity, similarity, repeatability, and heritability. International Journal of Comparative Psychology 17, 304–314 (2004)

Paweletz, N. Walther Flemming: Pioneer of mitosis research. Nature Reviews Molecular Cell Biology 2, 72–75 (2001) doi:10.1038/35048077 (link to article)

What is the difference between the cells that result from mitosis and the cells that result from meiosis quizlet?

What is the difference between the cells that result from mitosis?

What are the 3 main differences between mitosis and meiosis?

What are two major differences between mitosis and meiosis?